Why Standardization Is Critical in Immunophenotyping

In episode 28 of “The Conversational Flow”, our hosts, Adam and Brian, discuss why standardization is essential for credible flow cytometry data at scale. They walk through the biggest sources of variability - reagents and lots, sample handling and shipping time, anticoagulants and processing choices (whole blood vs. PBMCs, fresh vs. cryopreserved), instrument setup, and downstream analysis - and explain how each one can create “noise” that masquerades as biology. They note practical realitie...