Considerations for Flow Cytometry-based Cell Sorting

In this episode, Adam and Brian explored recent advances in cell sorting technology, focusing on image-activated approaches. Unlike traditional flow cytometry, which relies on voltage signals, these systems capture and analyze cell images in real time to guide sorting decisions, enabling new insights such as subcellular localization and cell-to-cell signaling. The hosts emphasized trade-offs between purity, yield, and speed, as well as technical considerations like nozzle size, sample stabili...